merck 539790-1KIT ProteoExtract Subcellular Proteome Extraction Kit ,ProteoExtr
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產品名稱: merck 539790-1KIT ProteoExtract Subcellular Proteome Extraction Kit ,ProteoExtr
產品型號: merck 539790-1KIT
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merck 539790-1KIT ProteoExtract Subcellular Proteome Extraction Kit ,ProteoExtract 亞細胞蛋白質組抽
merck 539790-1KIT ProteoExtract Subcellular Proteome Extraction Kit ,ProteoExtr
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merck 539790-1KIT ProteoExtract Subcellular Proteome Extraction Kit ,ProteoExtract 亞細胞蛋白質組抽
ProteoExtract 亞細胞蛋白質組抽提試劑盒
MERCK相關產品資料：
539790 ProteoExtract Subcellular Proteome Extraction Kit ( ProteoExtract® Subcellular Proteome Extraction Kit )
S-PEK Kit
Fast and reproducible extraction of subcellular proteomes from mammalian cells
ProteoExtract Subcellular Proteome Extraction Kit (S-PEK) is designed for fast and reproducible extraction of subcellular proteomes from adherent and suspension-grown mammalian cells. The S-PEK takes advantage of the different solubilities of certain subcellular compartments in the four selected reagents. In the case of adherent cells, the procedure is performed directly in the tissue culture dish without the need for cell removal. Cells or the parts of the cells remain attached to the plate during sequential extraction of subcellular compartments, until the appropriate extraction reagent is used. Thus, the early destruction of the cellular structure by enzymatic or mechanical detachment of cells from the tissue culture plate and any mixing of different subcellular compartments is prevented. For suspension-grown cells, extraction starts with gentle sedimentation and washing of the cells. The stepwise extraction delivers four distinct protein fractions from one sample:
- Cytosolic fraction (F1)
- Membrane/organelle protein fraction (F2)
- Nucleic protein fraction (F3)
- Cytoskeletal fraction (F4)
Proteins are obtained in the native state making the S-PEK suitable for many downstream applications such as 1D and 2D gel electrophoresis, immunoblotting, enzyme activity assays, and protein microarrays.
Sample size: 3-5x106 or 25-50 mg tissue.
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Product number
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型號
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價格
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備注
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539790-1KIT
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1 kit
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詢價
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產品信息
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Form
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20 Extractions
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Avoid freeze/thaw
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Yes
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Sample type
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Mammalian tissue, cultured mammalian cells
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Kit contains
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Wash buffer, Extraction Buffer I, Extraction Buffer II, Extraction Buffer III, Extraction Buffer IV, Protease Inhibitor Cocktail, Benzonase® Nuclease (Cat. No. 71206), and a user protocol.
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Features and benefits
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- Stepwise extraction resulting in four distinct subcellular proteomes from one sample
- Highly reproducible
- No ultracentrifugation steps
- Fast—needs just 2 hours with 45 minutes hands-on time
- Produces proteins suitable for functional studies
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Store and ship information
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Storage
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+2°C to +8°C
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Ship
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Ambient Temperature Only
Multiple Toxicity Values, refer to MSDS
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**標示
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S Phrase
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S: 22-26-28.2-36/37-45
Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
After contact with skin, wash immediately with soap and plenty of water.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
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R Phrase
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R: 22-36/38-52/53
Harmful if swallowed.
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