產品名稱：Recombinant NFkB p65 protein
產品貨號：31102
供應商：Active Motif
產品規格：5 µg
Contents
5 µg of Recombinant NFκB p65 protein 1X Protein Dilution Buffer (20 mM Tris-HCl, 0.2 M NaCl, 2 mM MgCl2 and 10% glycerol) and 1 M DTT. Protein is supplied at 100 ng/µl in Protein Dilution Buffer.
Protein Details
Recombinant NFκB p65 protein was expressed from a full-length cDNA clone in E. coli and has a 14 amino acid truncation at the C-term. This clone had five point mutations compared to the p65 sequence listed under accession no. AAA36408: L159V, P180S, F309S, A439V and V462M. The protein was purified by affinity chromatography, followed by gel filtration
Application Notes
Recombinant NFκB p65 is suitable for Western blotting and TransAM® assays. 100 ng is sufficient for most protein-protein interaction studies. The standard curve for TransAM NFκB p65 was generated using the range of 40-0.625 ng of protein. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided. One unit equals 1 nanogram.

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