產品名稱：Recombinant p300 protein
產品貨號：31124
供應商：Active Motif
產品規格：4 µg
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Protein Details
The wild-type p300 protein was expressed in a baculovirus system with a flag (accession number NM 001429) and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 95% homogeneous and contains no detectable protease, DNase and RNase activity.
Application Notes
Recombinant p300 is suitable for protein-protein interaction, in vitro transcription, in vitro acetylation and cell growth assays. 1-5 ng is sufficient for gelshift assays in a 20 µl volume, 50-100 ng is sufficient for reconstituted transcription assays and 100-200 ng is sufficient for protein-protein interaction or acetylation assays. The molecular weight of the protein is ~300 kDa. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
Contents
4 µg of Recombinant p300 protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Protein is supplied at 0.2 µg/µl in Dilution Buffer AM1.

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