產品名稱：T3, free ELISA
產    地：Demeditec 
產品貨號：DE2385
產品規格：96 Tests
其它產品：
T4, free ELISA Demeditec DE2386 96 Tests 2156
產品說明：
Special remarks:
1 INTENDED USE
For the quantitative determination of Free Triiodothyronine (fT3) concentration in human Serum.
2 INTRODUCTION
L-Triiodothyronine, a thyroid hormone, circulates in blood almost completely bound (>99.5%) to carrier proteins.
The main transport protein is thyroxine-binding globulin (TBG).  However, only the free (unbound) portion of triiodothyronine is believed to be responsible for the biological action.  Furthermore, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In individuals with normal thyroid function, as the concentrations of the carrier proteins change, the total T3 levels change in consert so that the free thriiodothyronine (fT3) concentration remains constant.  Thus, measurements of fT3 concentrations correlate more reliably with clinical status than total triiodothyronine levels.
For example, the increase in total triiodothyronine levels associated with pregnancy, oral contraceptives, and estrogen therapy result in higher total T3 levels while the fT3 concentration remains basically unchanged. This microplate enzyme immunoassay methodology provides the technician with optimum sensitivity while requiring few technical manipulations in a direct determination of fT3
3 PRINCIPLE OF THE TEST
The fT3 test is a solid phase competitive enzyme immunoassay.  Patient serum samples, standards, and T3-Enzyme Conjugate Working Reagent is added to wells coated with monoclonal T3 antibody. fT3 in the patient specimen and the T3 labeled conjugate compete for available binding sites on the antibody.  After a 60 minutes incubation at room temperature, the wells are washed with water to remove unbound T3 conjugate.  A solution of H 2 O 2 /TMB is then added and incubated for 20 minutes, resulting in the development of blue color.  The color development is stopped with the addition of 3N HCl, and the absorbance is measured spectrophotometrically at 450 nm.  The intensity of the color formed is proportional to the amount of enzyme present and is inversely related to the amount of unlabeled fT3 in the sample.  By reference to a series of fT3 standards assayed in the same way, the concentration of fT3 in the unknown sample is quantified.
上海玉博生物技術有限公司在為生命科學領域提供豐富的產品與信息資源方面處于****地位，公司提供的產品涵蓋了二十多個國家近五十萬種產品，而且產品的數量與信息在不斷的增長和更新，公司提供的產品能夠使生命科學工作者加快對生物化學，分子生物學，細胞生物學以及蛋白質組學研究的認知，以及分子診斷和臨床醫學領域的應用。

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