產品名稱：Hepcidin Prohormone ELISA
產    地：Demeditec 
產品貨號：DE4644
產品規格：96 Tests
產品說明：
Special remarks:
1 Introduction
The DEMEDITEC Hepcidin Prohormone Enzyme Immunoassay Kit provides materials for the quantitative determination of Hepcidin Prohormone in human serum and urine.
This assay is intended for research use only.
Hepcidin is a small cystein-rich peptide produced in the liver [1]. This molecule regulates the absorption of iron in the body similarly to the correlation of insulin and sugar.
Hepcidin was initially isolated as a 25 amino acid (aa) peptide in human plasma and urine exibiting antimicrobial activity [2,3].
Human Hepcidin is produced from a 84 aa precursor including a putative (24 aa) signal peptide.
Application of the present ELISA allows the detection and determination of Hepcidin Prohormone (Pro?Hepcidin) in serum of patients suffering from several disorders of iron metabolism.
2 Principle of the test
The DEMEDITEC Hepcidin Prohormone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding.
The microtiter wells are coated with a polyclonal antibody directed towards an antigenic site on the Hepcidin Prohormone molecule (28-47 aa). Endogenous Hepcidin Prohormone of a patient sample competes with a Hepcidin Prohormone-biotin conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off.
The amount of bound biotin conjugate is reverse proportional to the concentration of Hepcidin Prohormone in the sample. After addition of the substrate solution, the intensity of colour developed is reverse proportional to the concentration of Hepcidin Prohormone in the patient sample.
上海玉博生物技術有限公司在為生命科學領域提供豐富的產品與信息資源方面處于****地位，公司提供的產品涵蓋了二十多個國家近五十萬種產品，而且產品的數量與信息在不斷的增長和更新，公司提供的產品能夠使生命科學工作者加快對生物化學，分子生物學，細胞生物學以及蛋白質組學研究的認知，以及分子診斷和臨床醫學領域的應用。

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