產品名稱：Involucrin specific components
產    地：Demeditec
產品貨號：DEBT650
產品規格：24x96 Tests
產品說明：
Special remarks: This is not a complete kit; it just contains the specific compomemts and has to be completed with commercial reagents.
Involucrin is a recently recognized structural component of mature squamous epithelial cells. We examined involucrin expression using an immunoperoxidase technique in normal skin and in a variety of epidermal hyperplasias and neoplasms to determine whether distinctive staining patterns existed within these lesions. Four patterns of reactivity were observed: diffuse intracellular staining typical of keratinocytes of the upper third of normal epidermis and epidermal hyperplasias and benign neoplasms; staining at cell borders, seen principally in benign epidermal neoplasms; patchy staining characteristic of squamous cell carcinoma in situ; and absence of staining in benign and neoplastic basaloid epithelium. Invasive nests of squamous cell carcinomas were negative for involucrin reactivity, whereas pseudoinvasive tongues of epithelium at the bases of keratoacanthomas were focally positive. These results suggest that immunoperoxidase staining for involucrin may be useful in distinguishing certain benign from malignant epidermal neoplasms as well as in understanding the altered maturation and kinetics of proliferative processes afflicting keratinocytes.
Involucrin, a soluble cytoplasmic protein with a highly repetitive amino acid sequence, serves as a substrate for transglutaminase cross-linking in the formation of cornified envelopes.  Like the cross linked envelope, involucrin expression is a specific marker for keratinocyte differentiation.  This protein is synthesized not only by cells of the epidermis and other squamous epithelia, but also in epithelia undergoing squamous metaplasia and in certain tumors, as in the lung, which display epidermoid characteristics.  In culture, the growth and differentiation of keratinocytes, especially those derived from tumors, can be modulated greatly by  physiological (retinoids, calcium) and toxic agents (including  carcinogens). The involucrin content of the cells is highly responsive to such treatments and thus its measurement by ELISA can serve as a convenient and sensitive indicator of the cellular response.
This Involucrin ELISA Kit is designed to provide a reproducible, quantitative method of measuring Involucrin. The assay is highly sensitive, 0.05-0.5 nanograms per well.  Levels of Involucrin can be directly measured after separation of cellular debris.  The kit includes purified human Involucrin, a highly specific rabbit anti-human Involucrin and an affinity purified goat anti-rabbit IgG alkaline phosphatase.  Sufficient quantities of these reagents are provided for approximately 24 microtiter plates.
上海玉博生物技術有限公司在為生命科學領域提供豐富的產品與信息資源方面處于****地位，公司提供的產品涵蓋了二十多個國家近五十萬種產品，而且產品的數量與信息在不斷的增長和更新，公司提供的產品能夠使生命科學工作者加快對生物化學，分子生物學，細胞生物學以及蛋白質組學研究的認知，以及分子診斷和臨床醫學領域的應用。

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