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Normetanephrine Plasma Fast Track

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產(chǎn)品名稱: Normetanephrine Plasma Fast Track
產(chǎn)品型號(hào): DEE8200
產(chǎn)品展商: 原裝進(jìn)口
產(chǎn)品文檔: 無相關(guān)文檔

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Normetanephrine Plasma Fast Track


Normetanephrine Plasma Fast Track  的詳細(xì)介紹
Normetanephrine Plasma Fast Track

產(chǎn)品名稱:Normetanephrine Plasma Fast Track
產(chǎn)    地:Demeditec
產(chǎn)品貨號(hào):DEE8200
產(chǎn)品規(guī)格:96 Tests
產(chǎn)品說明:
Special remarks:
Intended use and principle of the test
Enzyme Immunoassay for the quantitative determination of free Normetanephrine in plasma.
First, the plasma proteins are removed by precipitation. After this Normetanephrine (Normetadrenaline) is quantitatively acylated.
The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.
Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.
The antisera used in this test kit only recognise the biologically relevant L-forms of metanephrines. Commercially available synthetic normetanephrine or metanephrine is always a mixture of the D- and L-forms. The ratio between both forms differs widely from lot to lot. This has important implications if synthetic metanephrines are used to enrich native samples. As only about 50% of the synthetic metanephrines - the L-portion - will be detected by use of this kit, spiked samples will be underestimated. Therefore native samples containing solely the L-form should be used.
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