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2 MET Plasma Fast Track(Metanephrin + Normetanephrin)

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產品名稱: 2 MET Plasma Fast Track(Metanephrin + Normetanephrin)
產品型號: DEE8300
產品展商: 原裝進口
產品文檔: 無相關文檔

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2 MET Plasma Fast Track(Metanephrin + Normetanephrin)


2 MET Plasma Fast Track(Metanephrin + Normetanephrin)  的詳細介紹
2 MET Plasma Fast Track(Metanephrin + Normetanephrin)

產品名稱:2 MET Plasma Fast Track(Metanephrin + Normetanephrin)
產    地:Demeditec 
產品貨號:DEE8300
產品規格:2x96 Tests
產品說明:
Special remarks:
Intended use and principle of the test
Enzyme Immunoassay for the quantitative determination of free Metanephrine and free Normetanephrine in plasma.
First, the plasma proteins are removed by precipitation. After this Metanephrine (Metadrenaline) and Normetanephrine (Normetadrenaline) are quantitatively acylated.
The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.
Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.
The antisera used in this test kit only recognise the biologically relevant L-forms of metanephrines. Commercially available synthetic normetanephrine or metanephrine is always a mixture of the D- and L-forms. The ratio between both forms differs widely from lot to lot. This has important implications if synthetic metanephrines are used to enrich native samples. As only about 50% of the synthetic metanephrines - the L-portion - will be detected by use of this kit, spiked samples will be underestimated. Therefore native samples containing solely the L-form should be used.
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