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Anti-GAD RIA

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產品名稱: Anti-GAD RIA
產品型號: DEGAD100
產品展商: 原裝進口
產品文檔: 無相關文檔

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Anti-GAD RIA


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Anti-GAD RIA

產品名稱:Anti-GAD RIA
產    地:Demeditec 
產品貨號:DEGAD100
產品規格:100 Tests
產品說明:
Special remarks:
INTENDED USE
The DEMEDITEC GAD autoantibody assay kit is intended for use by professional persons only, for the quantitative determination of GAD autoantibodies (GAD Ab) in human serum.
Autoantibodies to pancreatic beta cell antigens are important serological markers of type 1 diabetes mellitus. The antigens recognised by these antibodies include insulin, glutamic acid decarboxylase (GAD65 kDa isoform) and the islet cell antigen IA2 or ICA512.
ASSAY PRINCIPLE
In DEMEDITEC’s GADAb radioimmunoassay (RIA), GAD antibodies in patient’s sera, calibrators and controls are allowed to interact with 125I labelled GAD65. After a 2 hour incubation, solid phase protein A is added to precipitate labelled GAD-GAD autoantibody complexes. After a further 1 hour incubation period, assay buffer is added to each tube and each tube then centrifuged. Any unbound 125I is removed from the tubes by aspiration of the supernatants. The pellets are then counted on a gamma counter and the % binding determined. The level of radioactivity in the sample is proportional to the amount of GAD antibody in the sample. The measuring range is 1 – 300 U/mL.
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