現(xiàn)貨 R&D貨號(hào)M6000B Mouse IL-6 Quantikine ELISA Kit
特價(jià) 原裝進(jìn)口 小鼠白介素6 elisa試劑盒；5000元，現(xiàn)貨促銷
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Assay Length
4.5 hours
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Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), Heparin Plasma (50 uL)
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Sensitivity
1.8 pg/mL
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Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
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Specificity
Natural and recombinant mouse IL-6
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Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
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Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse IL-6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-6. Results obtained using natural mouse IL-6 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-6.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
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2
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3
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n
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20
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20
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20
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28
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22
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27
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Mean
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30
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87
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232
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29
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85
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238
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Standard Deviation
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2
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3.4
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8.1
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1.8
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7.5
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18.1
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CV%
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6.7
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3.9
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3.5
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6.1
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8.9
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7.6
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Recovery
The recovery of mouse IL-6 spiked to three levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Cell Culture Supernates (n=5)
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110
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86-120
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Heparin Plasma (n=6)
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101
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81-115
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Serum (n=5)
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99
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84-113
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-6 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Background: IL-6
IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.
